Changing molecular epidemiology of Hepatitis A virus in Santiago, Chile from 2010 to 2021.

OBJECTIVES: Hepatitis A (HAV) virus causes asymptomatic to life-treating fulminant hepatitis. During infection, patients show large viral excretion in their stools. Resistance of HAV to environmental conditions, allows us to recover viral nucleotide sequences from wastewater and trace its evolutionary history. METHODS: We characterize twelve years of HAV circulation in wastewater from Santiago, Chile, and conducted phylogenetic analyses to decipher the dynamics of circulating lineages. RESULTS: We observed the exclusive circulation of the HAV IA genotype. The molecular epidemiologic analyses showed a steady circulation of a dominant lineage with low genetic diversity (d = 0,007) between 2010 and 2017. An outbreak of Hepatitis A associated with men who have sex with men, in 2017 was associated with the irruption of a new lineage. Remarkably, a dramatic change in the dynamic of HAV circulation was observed in the period post-outbreak; between 2017 and 2021 when 4 different lineages were transiently detected. Exhaustive phylogenetic analyses indicate that these lineages were introduced and possibly derived from isolates from other Latin American countries. CONCLUSION: The HAV circulation in recent years in Chile is rapidly changing and suggests that this phenomenon could be a consequence of massive population migrations in Latin America caused by political instability and natural disasters.

Physicochemical properties of three bioceramic cements.

This study aimed to compare the physicochemical properties of MTA Angelus (MTA-A), MTA Repair HP (MTA-HP), and Biodentine (BD). Setting times (n = 7) were determined in accordance with ASTM C266-15. Solubility (n = 11), pH (n = 10), and calcium ion release (n = 10) were evaluated up to 28 days in accordance with ANSI/ADA specification no. 57. Radiopacity was assessed by ANSI/ADA (n = 10) and the tissue simulator method (n = 10). In both methods, the specimens were radiographed using an aluminum stepwedge and the digital radiographs were analyzed in Adobe Photoshop, determining the mean grayscale pixel values of the materials, of the 3-mm aluminum stepwedge, and of the dentin, the latter of which was analyzed on the tissue simulator. The data obtained from each test were statistically analyzed and compared (p < 0.05). MTA-A presented longer final setting time compared with the other materials. There were no significant differences in the mass values of materials during the experiment. All materials presented an alkaline pH. BD promoted greater calcium ion release in most of the experimental periods. All materials presented appropriate radiopacity. BD showed lower radiopacity than MTA-A in the tissue simulator method. All groups presented higher radiopacity in the tissue simulator when compared with the ANSI/ADA method. MTA-A, MTA-HP, and BD showed appropriate physicochemical properties and radiopacity, and were considered suitable to be used in clinical practice.

Physicochemical properties of three bioceramic cements

Abstract: This study aimed to compare the physicochemical properties of MTA Angelus (MTA-A), MTA Repair HP (MTA-HP), and Biodentine (BD). Setting times (n = 7) were determined in accordance with ASTM C266-15. Solubility (n = 11), pH (n = 10), and calcium ion release (n = 10) were evaluated up to 28 days in accordance with ANSI/ADA specification no. 57. Radiopacity was assessed by ANSI/ADA (n = 10) and the tissue simulator method (n = 10). In both methods, the specimens were radiographed using an aluminum stepwedge and the digital radiographs were analyzed in Adobe Photoshop, determining the mean grayscale pixel values of the materials, of the 3-mm aluminum stepwedge, and of the dentin, the latter of which was analyzed on the tissue simulator. The data obtained from each test were statistically analyzed and compared (p < 0.05). MTA-A presented longer final setting time compared with the other materials. There were no significant differences in the mass values of materials during the experiment. All materials presented an alkaline pH. BD promoted greater calcium ion release in most of the experimental periods. All materials presented appropriate radiopacity. BD showed lower radiopacity than MTA-A in the tissue simulator method. All groups presented higher radiopacity in the tissue simulator when compared with the ANSI/ADA method. MTA-A, MTA-HP, and BD showed appropriate physicochemical properties and radiopacity, and were considered suitable to be used in clinical practice.

Equipes de saúde bucal na estratégia da saúde da família em polo urbano do sul do Brasil / Oral health teams in the family health strategy in urban pole of southern Brazil

Introdução: a Estratégia da Saúde da Família (ESF) é uma política brasileira recente de gestão e execução dos serviços de saúde. Um dos alvos desta política é promover a saúde bucal. Para tanto, as equipes devem ser constituídas segundo normas do Ministério da Saúde, compostas por cirurgião-dentista e pelo menos um auxiliar. Objetivo: avaliar a composição das equipes de saúde bucal da ESF do município de Passo Fundo/RS/Brasil e comparar com as normas do Ministério da Saúde. Métodos: neste estudo transversal, a composição de 22 ESFs foi avaliada através de consulta ao Sistema de Informação de Atenção Básica (SIAB), referente ao biênio 2013-2014, e por aplicação de questionário para avaliar a estrutura das Equipes da Saúde da Família. Resultados: nenhuma das 22 ESFs apresentou equipe de saúde bucal completa. O único profissional identificado foi o cirurgião-dentista, presente em 17 das 22 ESFs (77%), integralmente pago com verba municipal, sem contrapartida federal. Não há uma padronização na carga horária do profissional odontólogo, diferente dos demais profissionais que compõem a atenção básica. Das ESFs regularizadas no SIAB, 20% não apresentam cirurgião-dentista em sua composição, ficando esta população desassistida em saúde bucal. Conclusões: as equipes de saúde bucal das ESFs do município de Passo Fundo/RS são incompletas e não seguem as normas Brasileiras.

Introduction: the Family Health Strategy (ESF) is a recent Brazilian policy of management and execution from health services. One of its targets is to promote the Oral Health. In order to achieve this the staffs shall be established accordingly to the Health Ministry rules, formed by a dental surgeon and at least one assistant. Objective: to evaluate the oral health teams structure of the ESF in the city of Passo Fundo/ RS/Brazil and contrast it with the Health Ministry rules. Methods: in this cross-sectional study, were valued the structure of 22 ESFs by looking at the Basic Health Care Information System (SIAB), referring to the biennium 2013-2014 and by applying a questionnaire to evaluate the ESF´s teams structure. Results: none of the 22 ESFs reported an Oral Health perfect team. The only professional identified was the dental surgeon, existing in 17 out of 22 ESFs (77%), paid in full by municipal funds with no federal financial reward. There is not a standard at dentists workload oppositely to other professional in SIAB. From the ESFs settled at SIAB 20% have no dental surgeon in its staff leaving people unattended in oral health. Conclusions: the oral health ESFs teams from the city of Passo Fundo/RS are incomplete and don´t obey the Brazilian regulation.

Uma reflexão sobre a atenção básica no sistema único da saúde / A reflection on primary care in the unified health system

Introdução: o presente trabalho aborda o tema "O papel da atenção básica no Sistema Único de Saúde". A qualidade dos serviços de saúde remete à necessidade da adoção de um modelo de organização em saúde, como forma de otimizar os serviços de saúde pública, tornando-os mais eficientes. Objetivo: o objetivo deste trabalho é realizar uma revisão da literatura sobre os desafios da atenção primária e a implementação da Estratégia Saúde da Família no Brasil. Métodos: o estudo foi realizado a partir de pesquisa bibliográfica, que permitiu abordar o tema, fundamentando-o em fontes como livros, periódicos, artigos publicados e consulta a meios eletrônicos. Resultados e conclusão: concluiu-se que oferecer atenção à saúde frente às inovações da tecnologia não basta. É preciso também introduzir um processo racional e, sobretudo, bom senso no desempenho diário das equipes de saúde e da gestão.

Introduction: the current study approaches the role of primary care in the "Unified Health System". The quality of health services refers to the necessity of adopting a model of organization in health, in the order to optimize the public health service, making them more efficient. Objective: the objective is to review the literature on the challenges of primary care and the implementation of the Family Health Program in Brazil. Methods: the methodology used was carried out through a literature review, which allowed to analyze the issue, based on sources as books, periodicals, published articles and consulting to electronic media. Results and Conclusion: it was concluded that providing a health care considering the innovations of technology is not enough. It must also be introduced a rational process, and especially, good sense in the daily performance of the health staff and management.

Uma reflexão sobre a atenção básica no Sistema Único de Saúde

O presente trabalho aborda o tema "O papel da atenção básica no Sistema Único de Saúde". A qualidade dos serviços de saúde remete à necessidade da adoção de um modelo de organização em saúde, como forma de otimizar os serviços de saúde pública, tornando-os mais eficientes. O objetivo deste trabalho é fazer uma revisão da literatura sobre os desafios da atenção primária e a implementação da Estratégia Saúde da Família no Brasil. A metodologia utilizada, neste trabalho, foi realizada a partir de pesquisa bibliográfica, que permitiu abordar o tema, fundamentando-o em fontes como livros, periódicos, artigos publicados e consulta a meios eletrônicos. Concluiu-se que oferecer atenção à saúde frente às inovações da tecnologia não basta. É preciso também introduzir um processo racional e, sobretudo, bom senso no desempenho diário das equipes de saúde e da gestão.